Enzyme assisted extraction of chitin from shrimp shells (Litopenaeus vannamei)

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Enzyme assisted extraction of chitin from shrimp shells (Litopenaeus vannamei)

8 BACKGROUND: Chemical chitin extraction generates large amounts of wastes and increases partial 9 deacetylation of the product. Therefore, the use of biological methods for chitin extraction is an 10 interesting alternative. The effects of process conditions on enzyme assisted extraction of chitin from 11 the shrimp shells in a systematic way were the focal points of this study. 12 RESULTS: De...

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Preparation and Characterization of Chitosan Obtained from Shells of Shrimp (Litopenaeus vannamei Boone)

The main source of commercial chitosan is the extensive deacetylation of its parent polymer chitin. It is present in green algae, the cell walls or fungi and in the exoskeleton of crustaceans. A novel procedure for preparing chitosan from shrimp shells was developed. The procedure involves two 10-minutes bleaching steps with ethanol after the usual demineralization and deproteinization processe...

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Microbiota from Litopenaeus vannamei: digestive tract microbial community of Pacific white shrimp (Litopenaeus vannamei)

Bacteria capable of producing different extracellular enzymes of potential relevance in digestive processes were isolated from the stomach, hepatopancreas and intestine of Pacific white shrimp Litopenaeus vannamei. A total of 64 strains with proteolytic activity were isolated and grouped into 16 clusters based on morphological characteristics: 4 groups were isolated from the intestine; 5 from t...

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Cuticular chitin synthase and chitinase mRNA of whiteleg shrimp Litopenaeus vannamei during the molting cycle

Cuticular chitin synthase and chitinase mRNA of whiteleg shrimp Litopenaeus vannamei during the molting cycle a b s t r a c t a r t i c l e i n f o Chitin metabolism is of high relevance for shrimp growth because it has to be synthesized and cleaved in each molt. We studied chitin synthase and chitinase mRNAs from whiteleg shrimp Litopenaeus vannamei. For this, cDNA coding for cuticular chitin ...

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The protective effect of chitin and chitosan against Vibrio alginolyticus in white shrimp Litopenaeus vannamei.

White shrimp, Litopenaeus vannamei, which had been injected with chitin at 4, 6 and 8 microg g(-1) or chitosan at 2, 4 and 6 microg g(-1), were challenged with pathogen Vibrio alginolyticus at 2 x 10(6) colony-forming units (cfu) shrimp(-1) and then placed in seawater of 34 per thousand. The survival of shrimp that received chitin or chitosan at either dose was significantly higher than that of...

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ژورنال

عنوان ژورنال: Journal of Chemical Technology & Biotechnology

سال: 2015

ISSN: 0268-2575

DOI: 10.1002/jctb.4714